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perm-s buffer fluidigm
Perm S Buffer Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer+fluidigm/perm+s+buffer+fluidigm/pm40505659-504-13-15
Average 90 stars, based on 1 article reviews
Perm S Buffer Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer+fluidigm/perm+s+buffer+fluidigm/pm40505659-504-13-15
Average 90 stars, based on 1 article reviews
perm-s buffer fluidigm - by Bioz Stars,
2026-10
90/100 stars
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Red Blood Cell Lysis:Article Title: Fecal microbiota transplantation improves anti-PD-1 inhibitor efficacy in unresectable or metastatic solid cancers refractory to anti-PD-1 inhibitor. Article Snippet: In brief Kim et al. show that fecal microbiota transplantation combined with anti-PD-1 therapy can overcome resistance in advanced solid cancers.. They identify Prevotella merdae Immunoactis, which enhances immune response and inhibits tumor growth, while confirming two nonefficacious strains that may inhibit T cell activity, suggesting that specific microbiota could improve immunotherapy effectiveness. Staining:Article Title: Fecal microbiota transplantation improves anti-PD-1 inhibitor efficacy in unresectable or metastatic solid cancers refractory to anti-PD-1 inhibitor. Article Snippet: In brief Kim et al. show that fecal microbiota transplantation combined with anti-PD-1 therapy can overcome resistance in advanced solid cancers.. They identify Prevotella merdae Immunoactis, which enhances immune response and inhibits tumor growth, while confirming two nonefficacious strains that may inhibit T cell activity, suggesting that specific microbiota could improve immunotherapy effectiveness. Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003, RRID: AB_10015289 Peroxidase-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgM Jackson ImmunoResearch Cat# 115-036-020, RRID: AB_2338521 Peroxidase-conjugated AffiniPure goat anti-human IgG (H + L) Jackson ImmunoResearch Cat# 109-035-003, RRID: AB_2337577 R-phycoerythrin-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-116-071, RRID: AB_2338626 Recombinant anti-b actin antibody (Mouse) Servicebio Cat# GB15001, RRID: AB_3083704 Anti-human MICA/B a3 antibody (Mouse, Clone: MIA-2) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-16) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-18) This paper N/A InVivoMab anti-mouse CD4 (Clone: GK1.5) BioXcell Cat# BE0003, RRID: AB_1107642 InVivoMab anti-mouse CD8a (Clone: 2.43) BioXcell Cat# BE0061, RRID: AB_1125541 InVivoMab anti-mouse NK1.1 (Clone: PK136) BioXcell Cat# BE0036, RRID: AB_1107737 InVivoMab anti-mouse B220 (Clone: RA3.3A1/6.1 (TIB-146)) BioXcell Cat# BE0067, RRID: AB_1107651 APC anti-human MICA/B (Clone: 6D4) BioLegend Cat# 320907, RRID: AB_493196 FITC anti-human CD326 (EpCAM) (Clone: 9C4) BioLegend Cat# 324203, RRID: AB_756077 PE/Cyanine 7 anti-mouse CD45 (Clone: 30-F11) BioLegend Cat# 103114, RRID: AB_312979 APC/Cyanine7 anti-mouse CD4 (Clone: RM4-5) BioLegend Cat# 100526, RRID: AB_312727 Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2) BioLegend Cat# 100216, RRID: AB_493697 PerCP/Cyanine5.5 anti-mouse CD8a (Clone: 53-6.7) BioLegend Cat# 100734, RRID: AB_2075238 APC anti-mouse NK1.1 (Clone: PK136) BD Pharmingen Cat# 550627, RRID: AB_398463 PE-CF594 anti-mouse IFN-g (Clone: XMG1.2) BD Pharmingen Cat# 562303, RRID: AB_11153140 Brilliant Violet 421TM anti-human/mouse Granzyme B (Clone: QA18A28) BioLegend Cat# 396414, RRID: AB_2810603 FITC anti-human CD45 (Clone: HI30) BioLegend Cat# 982316, RRID: AB_2876779 APC anti-human CD19 (Clone: HIB19) BioLegend Cat# 982406, RRID: AB_2650645 PerCP/Cyanine5.5 anti-human CD45 (Clone: HI30) BioLegend Cat# 304028, RRID: AB_893338 Brilliant Violet 605TM anti-human CD3 (Clone: UCHT1) BioLegend Cat# 300459, RRID: AB_2564379 FITC anti-human CD4 (Clone: SK3) BioLegend Cat# 344604, RRID: AB_1937227 APC/Cyanine7 anti-human CD8 (Clone: SK1) BioLegend Cat# 344714, RRID: AB_2044006 APC anti-human IFN-g (Clone: 4S.B3) BioLegend Cat# 502512, RRID: AB_315237 (Continued on next page) Cell Reports Medicine 6, 101981, March 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lysozyme Sangon Biotech Cat# A610308 Recombinant human MICA protein (C-6His) Novoprotein Cat# C489 Horseradish peroxidase-labeled streptavidin Beyotime Cat# A0305 LipofectamineTM 3000 Invitrogen Cat# L3000015 D-luciferin potassium salt Yeasen Cat# 40902ES03 Ficoll-Hypaque separation solution TBD Cat# LTS1077 Recombinant human IL-2 Novoprotein Cat# C013 ACK lysis buffer Beyotime Cat# C3702 BV510-Zombie Dye BioLegend Cat# 423102 Fixation Buffer BioLegend Cat# 420801 Intracellular Staining Perm Wash Buffer BioLegend Cat# 421002 Cisplatin Fluidigm Cat# 201064 Maxpar Fix and Sample Prep:Article Title: Fecal microbiota transplantation improves anti-PD-1 inhibitor efficacy in unresectable or metastatic solid cancers refractory to anti-PD-1 inhibitor. Article Snippet: In brief Kim et al. show that fecal microbiota transplantation combined with anti-PD-1 therapy can overcome resistance in advanced solid cancers.. They identify Prevotella merdae Immunoactis, which enhances immune response and inhibits tumor growth, while confirming two nonefficacious strains that may inhibit T cell activity, suggesting that specific microbiota could improve immunotherapy effectiveness. Cell Isolation:Article Title: Fecal microbiota transplantation improves anti-PD-1 inhibitor efficacy in unresectable or metastatic solid cancers refractory to anti-PD-1 inhibitor. Article Snippet: In brief Kim et al. show that fecal microbiota transplantation combined with anti-PD-1 therapy can overcome resistance in advanced solid cancers.. They identify Prevotella merdae Immunoactis, which enhances immune response and inhibits tumor growth, while confirming two nonefficacious strains that may inhibit T cell activity, suggesting that specific microbiota could improve immunotherapy effectiveness. Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003, RRID: AB_10015289 Peroxidase-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgM Jackson ImmunoResearch Cat# 115-036-020, RRID: AB_2338521 Peroxidase-conjugated AffiniPure goat anti-human IgG (H + L) Jackson ImmunoResearch Cat# 109-035-003, RRID: AB_2337577 R-phycoerythrin-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-116-071, RRID: AB_2338626 Recombinant anti-b actin antibody (Mouse) Servicebio Cat# GB15001, RRID: AB_3083704 Anti-human MICA/B a3 antibody (Mouse, Clone: MIA-2) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-16) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-18) This paper N/A InVivoMab anti-mouse CD4 (Clone: GK1.5) BioXcell Cat# BE0003, RRID: AB_1107642 InVivoMab anti-mouse CD8a (Clone: 2.43) BioXcell Cat# BE0061, RRID: AB_1125541 InVivoMab anti-mouse NK1.1 (Clone: PK136) BioXcell Cat# BE0036, RRID: AB_1107737 InVivoMab anti-mouse B220 (Clone: RA3.3A1/6.1 (TIB-146)) BioXcell Cat# BE0067, RRID: AB_1107651 APC anti-human MICA/B (Clone: 6D4) BioLegend Cat# 320907, RRID: AB_493196 FITC anti-human CD326 (EpCAM) (Clone: 9C4) BioLegend Cat# 324203, RRID: AB_756077 PE/Cyanine 7 anti-mouse CD45 (Clone: 30-F11) BioLegend Cat# 103114, RRID: AB_312979 APC/Cyanine7 anti-mouse CD4 (Clone: RM4-5) BioLegend Cat# 100526, RRID: AB_312727 Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2) BioLegend Cat# 100216, RRID: AB_493697 PerCP/Cyanine5.5 anti-mouse CD8a (Clone: 53-6.7) BioLegend Cat# 100734, RRID: AB_2075238 APC anti-mouse NK1.1 (Clone: PK136) BD Pharmingen Cat# 550627, RRID: AB_398463 PE-CF594 anti-mouse IFN-g (Clone: XMG1.2) BD Pharmingen Cat# 562303, RRID: AB_11153140 Brilliant Violet 421TM anti-human/mouse Granzyme B (Clone: QA18A28) BioLegend Cat# 396414, RRID: AB_2810603 FITC anti-human CD45 (Clone: HI30) BioLegend Cat# 982316, RRID: AB_2876779 APC anti-human CD19 (Clone: HIB19) BioLegend Cat# 982406, RRID: AB_2650645 PerCP/Cyanine5.5 anti-human CD45 (Clone: HI30) BioLegend Cat# 304028, RRID: AB_893338 Brilliant Violet 605TM anti-human CD3 (Clone: UCHT1) BioLegend Cat# 300459, RRID: AB_2564379 FITC anti-human CD4 (Clone: SK3) BioLegend Cat# 344604, RRID: AB_1937227 APC/Cyanine7 anti-human CD8 (Clone: SK1) BioLegend Cat# 344714, RRID: AB_2044006 APC anti-human IFN-g (Clone: 4S.B3) BioLegend Cat# 502512, RRID: AB_315237 (Continued on next page) Cell Reports Medicine 6, 101981, March 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lysozyme Sangon Biotech Cat# A610308 Recombinant human MICA protein (C-6His) Novoprotein Cat# C489 Horseradish peroxidase-labeled streptavidin Beyotime Cat# A0305 LipofectamineTM 3000 Invitrogen Cat# L3000015 D-luciferin potassium salt Yeasen Cat# 40902ES03 Ficoll-Hypaque separation solution TBD Cat# LTS1077 Recombinant human IL-2 Novoprotein Cat# C013 ACK lysis buffer Beyotime Cat# C3702 BV510-Zombie Dye BioLegend Cat# 423102 Fixation Buffer BioLegend Cat# 420801 Intracellular Staining Perm Wash Buffer BioLegend Cat# 421002 Cisplatin Fluidigm Cat# 201064 Maxpar Fix and other:Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA EGFP-tagged MICA (allele 001)- overexpressing recombinant lentiviral vector Cyagen Biosciences N/A EGFP-tagged MICADa1/2 (a1‒a2 domain deletion)-overexpressing recombinant lentiviral vector Cyagen Biosciences N/A MICA-pCMV6-XL5 (allele 001) mammalian expression plasmid OriGene Cat# sc120030 MICA a3-pUC57 E coli cloning plasmid OriGene N/A Software and algorithms GraphPad Prism 9.0 software GraphPad Software, Inc. https://graphpad.com/scientificsoftware/ prism/ FlowJo 10.8 Tree Star Inc. https://www.flowjo.com/solutions/flowjo ImageJ NIH https://imagej.net/ij Aniview BLT https://www.blt-imaging.com/ Other Website: IEDB NIAID https://www.iedb.org Website: SWISS-MODEL N/A https://swissmodel.expasy.org Website: NetMHCIIpan-4.0 DTU Health Tech https://services.healthtech.dtu.dk/ services/NetMHCIIpan-4.0/ Website: NetMHC-4.0 DTU Health Tech https://services.healthtech.dtu.dk/ services/NetMHC-4.0/ Database: IPD-IMGT/HLA N/A https://www.ebi.ac.uk/ipd/imgt/hla/ OPEN ACCESS Article Title: IRE1α silences dsRNA to prevent taxane-induced pyroptosis in triple-negative breast cancer. Article Snippet: Article IRE1a silences dsRNA to prevent taxane-induced pyroptosis in triple-negative breast cancer Article Title: Single-cell atlas reveals characteristic changes in intrahepatic HBV-specific leukocytes Article Snippet: GraphPad Prism (version 8.0) GraphPad Software Inc https://www.graphpad.com/ scientificsoftware/prism/ FlowJo (version7.6.1) FlowJo, LLC https://www.flowjo.com/ solutions/flowjo Cytobank Beckman, LLC https://premium.cytobank.cn/ cytobank/experiments MATLAB R2016a N/A https://www.mathworks.com/ Normalizer (1) https://github.com/nolanlab/b ead-normalization/releases R version 3.5 R Development Core Team https://www.r-project.org/ R Studio RStudio Team, 2015 https://www.rstudio.com/ FlowSOM (2) https://github.com/SofieVG/Fl owSOM Circlize (3) https://cran.r-project.org/web/ 44 packages/circlize/index.html MCF-data-analysis (4) https://github.com/hartmannfj / MCF-data-analysis CyTOF workflow (5) https://f1000research.com/art icles/6-748# t-SNE (6) https://github.com/jkrijthe/Rts ne One-SENSE (7) N/A flowStats N/A https://www.bioconductor.org / packages/release/bioc/html/fl owStats.html pheatmap N/A https://cran.r-project.org/web/ packages/pheatmap/index.ht ml TCGAbiolinks (8) https://bioconductor.org/pack ages/release/bioc/html/TCG Abiolinks.html flowCore (9) N/A flowWorkspaceData (10) N/A Hmisc N/A https://cran.r-project.org/web/ packages/Hmisc/index.html ggplot2 N/A https://cran.r-project.org/web/ 45 packages/ggplot2/index.html Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA EGFP-tagged MICA (allele 001)- overexpressing recombinant lentiviral vector Cyagen Biosciences N/A EGFP-tagged MICADa1/2 (a1‒a2 domain deletion)-overexpressing recombinant lentiviral vector Cyagen Biosciences N/A MICA-pCMV6-XL5 (allele 001) mammalian expression plasmid OriGene Cat# sc120030 MICA a3-pUC57 E coli cloning plasmid OriGene N/A Software and algorithms GraphPad Prism 9.0 software GraphPad Software, Inc. https://graphpad.com/scientificsoftware/ prism/ FlowJo 10.8 Tree Star Inc. https://www.flowjo.com/solutions/flowjo ImageJ NIH https://imagej.net/ij Aniview BLT https://www.blt-imaging.com/ Other Website: IEDB NIAID https://www.iedb.org Website: SWISS-MODEL N/A https://swissmodel.expasy.org Website: NetMHCIIpan-4.0 DTU Health Tech https://services.healthtech.dtu.dk/ services/NetMHCIIpan-4.0/ Website: NetMHC-4.0 DTU Health Tech https://services.healthtech.dtu.dk/ services/NetMHC-4.0/ Database: IPD-IMGT/HLA N/A https://www.ebi.ac.uk/ipd/imgt/hla/ Article ll OPEN ACCESS Article Title: Inhibiting intracellular CD28 in cancer cells enhances antitumor immunity and overcomes anti-PD-1 resistance via targeting PD-L1. Article Snippet: Article Control:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Purification:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Lysis:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003, RRID: AB_10015289 Peroxidase-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgM Jackson ImmunoResearch Cat# 115-036-020, RRID: AB_2338521 Peroxidase-conjugated AffiniPure goat anti-human IgG (H + L) Jackson ImmunoResearch Cat# 109-035-003, RRID: AB_2337577 R-phycoerythrin-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-116-071, RRID: AB_2338626 Recombinant anti-b actin antibody (Mouse) Servicebio Cat# GB15001, RRID: AB_3083704 Anti-human MICA/B a3 antibody (Mouse, Clone: MIA-2) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-16) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-18) This paper N/A InVivoMab anti-mouse CD4 (Clone: GK1.5) BioXcell Cat# BE0003, RRID: AB_1107642 InVivoMab anti-mouse CD8a (Clone: 2.43) BioXcell Cat# BE0061, RRID: AB_1125541 InVivoMab anti-mouse NK1.1 (Clone: PK136) BioXcell Cat# BE0036, RRID: AB_1107737 InVivoMab anti-mouse B220 (Clone: RA3.3A1/6.1 (TIB-146)) BioXcell Cat# BE0067, RRID: AB_1107651 APC anti-human MICA/B (Clone: 6D4) BioLegend Cat# 320907, RRID: AB_493196 FITC anti-human CD326 (EpCAM) (Clone: 9C4) BioLegend Cat# 324203, RRID: AB_756077 PE/Cyanine 7 anti-mouse CD45 (Clone: 30-F11) BioLegend Cat# 103114, RRID: AB_312979 APC/Cyanine7 anti-mouse CD4 (Clone: RM4-5) BioLegend Cat# 100526, RRID: AB_312727 Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2) BioLegend Cat# 100216, RRID: AB_493697 PerCP/Cyanine5.5 anti-mouse CD8a (Clone: 53-6.7) BioLegend Cat# 100734, RRID: AB_2075238 APC anti-mouse NK1.1 (Clone: PK136) BD Pharmingen Cat# 550627, RRID: AB_398463 PE-CF594 anti-mouse IFN-g (Clone: XMG1.2) BD Pharmingen Cat# 562303, RRID: AB_11153140 Brilliant Violet 421TM anti-human/mouse Granzyme B (Clone: QA18A28) BioLegend Cat# 396414, RRID: AB_2810603 FITC anti-human CD45 (Clone: HI30) BioLegend Cat# 982316, RRID: AB_2876779 APC anti-human CD19 (Clone: HIB19) BioLegend Cat# 982406, RRID: AB_2650645 PerCP/Cyanine5.5 anti-human CD45 (Clone: HI30) BioLegend Cat# 304028, RRID: AB_893338 Brilliant Violet 605TM anti-human CD3 (Clone: UCHT1) BioLegend Cat# 300459, RRID: AB_2564379 FITC anti-human CD4 (Clone: SK3) BioLegend Cat# 344604, RRID: AB_1937227 APC/Cyanine7 anti-human CD8 (Clone: SK1) BioLegend Cat# 344714, RRID: AB_2044006 APC anti-human IFN-g (Clone: 4S.B3) BioLegend Cat# 502512, RRID: AB_315237 (Continued on next page) Cell Reports Medicine 6, 101981, March 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lysozyme Sangon Biotech Cat# A610308 Recombinant human MICA protein (C-6His) Novoprotein Cat# C489 Horseradish peroxidase-labeled streptavidin Beyotime Cat# A0305 LipofectamineTM 3000 Invitrogen Cat# L3000015 D-luciferin potassium salt Yeasen Cat# 40902ES03 Ficoll-Hypaque separation solution TBD Cat# LTS1077 Recombinant human IL-2 Novoprotein Cat# C013 ACK lysis buffer Beyotime Cat# C3702 BV510-Zombie Dye BioLegend Cat# 423102 Fixation Buffer BioLegend Cat# 420801 Intracellular Staining Perm Wash Buffer BioLegend Cat# 421002 Cisplatin Fluidigm Cat# 201064 Maxpar Fix and Modification:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Enzyme-linked Immunosorbent Assay:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Cytotoxicity Assay:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Software:Article Title: T cell cascade regulation initiates systemic antitumor immunity through living drug factory of anti-PD-1/IL-12 engineered probiotics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD3 Antibody (Clone: 17A2) BioLegend Cat#100236 FITC anti-mouse CD8a Antibody (Clone: 53–6.7) BioLegend Cat#100706 APC anti-mouse CD8a Antibody (Clone: S18018E) BioLegend Cat#162305 FITC anti-mouse CD279 (PD-1) Antibody (Clone: 29F.1A12) BioLegend Cat#135213 APC anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505809 PE anti-mouse TNF-a Antibody (Clone: MP6-XT22) BioLegend Cat#506305 APC anti-mouse/human CD44 Antibody (Clone: IM7) BioLegend Cat#103011 PE anti-mouse CD62L Antibody (Clone: MEL-14) BioLegend Cat#104407 APC anti-mouse CD25 Antibody (Clone: PC61) BioLegend Cat#102011 PE anti-mouse FOXP3 Antibody (Clone: MF-14) BioLegend Cat#126403 FITC anti-mouse CD4 Antibody (Clone: GK1.5) BioLegend Cat#100406 Purified anti-mouse IFN-g Antibody (Clone: XMG1.2) BioLegend Cat#505801 CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1) Thermo Fisher Scientific Cat#14-5983-80 PD-1 Monoclonal Antibody (7A11B1) Thermo Fisher Scientific Cat#MA5-15780 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#32460 Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat#A16160 32 metal-conjugated antibodies for CyTOF Fluidigm Table S1 Bacterial and virus strains E. coli Nissle1917 MiaoLingBio Cat#T0147 Biological samples Mouse: C57BL/6J (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: BABL/C (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. N/A Mouse: C57BL/6N Rag1-knockout (6–10 weeks old) Shanghai Southern Model Biotechnology Co., Ltd. NM-KI-00069 Chemicals, peptides, and recombinant proteins Recombinant murine IL-12 p70 PeproTech Cat#210-12 Histamine Sigma-Aldrich Cat#Y0001779 Tris(2-carboxyethyl) phosphine (TCEP) Sigma-Aldrich Cat#C4706 1, 6-Hexanediol diacrylate (HDDA) Alfa Aesar Cat#043203 Mal-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-1404-3.4k Cy5.5-PEG3400-NH2 Xi’an Ruixi Biological Technology Cat#R-PF-0067 Sulfo-Cy7 NHS ester Xi’an Ruixi Biological Technology Cat# R-H-7109 Kanamycin Sigma-Aldrich Cat#420311 4-methoxyphenol (MEHQ) Sigma-Aldrich Cat#92264 Polyvinyl alcohol (PVA) Sigma-Aldrich Cat#363081 Recombinant mouse IL-2 (carrier-free) BioLegend Cat#575406 2-mercaptoethanol Thermo Fisher Scientific Cat#21985023 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Thermo Fisher Scientific Cat#15630130 GlutaMAXTM supplement Thermo Fisher Scientific Cat#35050061 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 (Continued on next page) Cell Reports 43, 114086, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) Invitrogen Cat#C1157 VivoGloTM Luciferin Promega Cat#P1043 InVivoPlus anti-mouse PD-1(aPD-1, CD279) BioXCell Cat#BP0273 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089 InVivoMAb anti-mouse CD4 BioXCell Cat#BE0119 InVivoMAb anti-mouse CD8a BioXCell Cat#BE0061 Ultra-LEAFTM Purified anti-mouse CD20 Antibody Biolegend Cat#152115 eBioscienceTM fixation/permeabilization solution Invitrogen Cat#00-5523-00 phenylmethanesulfonyl fluoride (PMSF) Beyotime Cat#ST2573-100g RIPA lysis buffer Beyotime Cat#P0013C Maxpar Cell Staining Buffer Fluidigm Cat#201068 Maxpar Fix and Recombinant:Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003, RRID: AB_10015289 Peroxidase-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgM Jackson ImmunoResearch Cat# 115-036-020, RRID: AB_2338521 Peroxidase-conjugated AffiniPure goat anti-human IgG (H + L) Jackson ImmunoResearch Cat# 109-035-003, RRID: AB_2337577 R-phycoerythrin-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-116-071, RRID: AB_2338626 Recombinant anti-b actin antibody (Mouse) Servicebio Cat# GB15001, RRID: AB_3083704 Anti-human MICA/B a3 antibody (Mouse, Clone: MIA-2) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-16) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-18) This paper N/A InVivoMab anti-mouse CD4 (Clone: GK1.5) BioXcell Cat# BE0003, RRID: AB_1107642 InVivoMab anti-mouse CD8a (Clone: 2.43) BioXcell Cat# BE0061, RRID: AB_1125541 InVivoMab anti-mouse NK1.1 (Clone: PK136) BioXcell Cat# BE0036, RRID: AB_1107737 InVivoMab anti-mouse B220 (Clone: RA3.3A1/6.1 (TIB-146)) BioXcell Cat# BE0067, RRID: AB_1107651 APC anti-human MICA/B (Clone: 6D4) BioLegend Cat# 320907, RRID: AB_493196 FITC anti-human CD326 (EpCAM) (Clone: 9C4) BioLegend Cat# 324203, RRID: AB_756077 PE/Cyanine 7 anti-mouse CD45 (Clone: 30-F11) BioLegend Cat# 103114, RRID: AB_312979 APC/Cyanine7 anti-mouse CD4 (Clone: RM4-5) BioLegend Cat# 100526, RRID: AB_312727 Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2) BioLegend Cat# 100216, RRID: AB_493697 PerCP/Cyanine5.5 anti-mouse CD8a (Clone: 53-6.7) BioLegend Cat# 100734, RRID: AB_2075238 APC anti-mouse NK1.1 (Clone: PK136) BD Pharmingen Cat# 550627, RRID: AB_398463 PE-CF594 anti-mouse IFN-g (Clone: XMG1.2) BD Pharmingen Cat# 562303, RRID: AB_11153140 Brilliant Violet 421TM anti-human/mouse Granzyme B (Clone: QA18A28) BioLegend Cat# 396414, RRID: AB_2810603 FITC anti-human CD45 (Clone: HI30) BioLegend Cat# 982316, RRID: AB_2876779 APC anti-human CD19 (Clone: HIB19) BioLegend Cat# 982406, RRID: AB_2650645 PerCP/Cyanine5.5 anti-human CD45 (Clone: HI30) BioLegend Cat# 304028, RRID: AB_893338 Brilliant Violet 605TM anti-human CD3 (Clone: UCHT1) BioLegend Cat# 300459, RRID: AB_2564379 FITC anti-human CD4 (Clone: SK3) BioLegend Cat# 344604, RRID: AB_1937227 APC/Cyanine7 anti-human CD8 (Clone: SK1) BioLegend Cat# 344714, RRID: AB_2044006 APC anti-human IFN-g (Clone: 4S.B3) BioLegend Cat# 502512, RRID: AB_315237 (Continued on next page) Cell Reports Medicine 6, 101981, March 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lysozyme Sangon Biotech Cat# A610308 Recombinant human MICA protein (C-6His) Novoprotein Cat# C489 Horseradish peroxidase-labeled streptavidin Beyotime Cat# A0305 LipofectamineTM 3000 Invitrogen Cat# L3000015 D-luciferin potassium salt Yeasen Cat# 40902ES03 Ficoll-Hypaque separation solution TBD Cat# LTS1077 Recombinant human IL-2 Novoprotein Cat# C013 ACK lysis buffer Beyotime Cat# C3702 BV510-Zombie Dye BioLegend Cat# 423102 Fixation Buffer BioLegend Cat# 420801 Intracellular Staining Perm Wash Buffer BioLegend Cat# 421002 Cisplatin Fluidigm Cat# 201064 Maxpar Fix and Activation Assay:Article Title: An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003, RRID: AB_10015289 Peroxidase-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgM Jackson ImmunoResearch Cat# 115-036-020, RRID: AB_2338521 Peroxidase-conjugated AffiniPure goat anti-human IgG (H + L) Jackson ImmunoResearch Cat# 109-035-003, RRID: AB_2337577 R-phycoerythrin-conjugated AffiniPure F(ab’)2 fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-116-071, RRID: AB_2338626 Recombinant anti-b actin antibody (Mouse) Servicebio Cat# GB15001, RRID: AB_3083704 Anti-human MICA/B a3 antibody (Mouse, Clone: MIA-2) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-16) This paper N/A Anti-human MICA/B a1/2 antibody (Mouse, Clone: MIA-18) This paper N/A InVivoMab anti-mouse CD4 (Clone: GK1.5) BioXcell Cat# BE0003, RRID: AB_1107642 InVivoMab anti-mouse CD8a (Clone: 2.43) BioXcell Cat# BE0061, RRID: AB_1125541 InVivoMab anti-mouse NK1.1 (Clone: PK136) BioXcell Cat# BE0036, RRID: AB_1107737 InVivoMab anti-mouse B220 (Clone: RA3.3A1/6.1 (TIB-146)) BioXcell Cat# BE0067, RRID: AB_1107651 APC anti-human MICA/B (Clone: 6D4) BioLegend Cat# 320907, RRID: AB_493196 FITC anti-human CD326 (EpCAM) (Clone: 9C4) BioLegend Cat# 324203, RRID: AB_756077 PE/Cyanine 7 anti-mouse CD45 (Clone: 30-F11) BioLegend Cat# 103114, RRID: AB_312979 APC/Cyanine7 anti-mouse CD4 (Clone: RM4-5) BioLegend Cat# 100526, RRID: AB_312727 Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2) BioLegend Cat# 100216, RRID: AB_493697 PerCP/Cyanine5.5 anti-mouse CD8a (Clone: 53-6.7) BioLegend Cat# 100734, RRID: AB_2075238 APC anti-mouse NK1.1 (Clone: PK136) BD Pharmingen Cat# 550627, RRID: AB_398463 PE-CF594 anti-mouse IFN-g (Clone: XMG1.2) BD Pharmingen Cat# 562303, RRID: AB_11153140 Brilliant Violet 421TM anti-human/mouse Granzyme B (Clone: QA18A28) BioLegend Cat# 396414, RRID: AB_2810603 FITC anti-human CD45 (Clone: HI30) BioLegend Cat# 982316, RRID: AB_2876779 APC anti-human CD19 (Clone: HIB19) BioLegend Cat# 982406, RRID: AB_2650645 PerCP/Cyanine5.5 anti-human CD45 (Clone: HI30) BioLegend Cat# 304028, RRID: AB_893338 Brilliant Violet 605TM anti-human CD3 (Clone: UCHT1) BioLegend Cat# 300459, RRID: AB_2564379 FITC anti-human CD4 (Clone: SK3) BioLegend Cat# 344604, RRID: AB_1937227 APC/Cyanine7 anti-human CD8 (Clone: SK1) BioLegend Cat# 344714, RRID: AB_2044006 APC anti-human IFN-g (Clone: 4S.B3) BioLegend Cat# 502512, RRID: AB_315237 (Continued on next page) Cell Reports Medicine 6, 101981, March 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lysozyme Sangon Biotech Cat# A610308 Recombinant human MICA protein (C-6His) Novoprotein Cat# C489 Horseradish peroxidase-labeled streptavidin Beyotime Cat# A0305 LipofectamineTM 3000 Invitrogen Cat# L3000015 D-luciferin potassium salt Yeasen Cat# 40902ES03 Ficoll-Hypaque separation solution TBD Cat# LTS1077 Recombinant human IL-2 Novoprotein Cat# C013 ACK lysis buffer Beyotime Cat# C3702 BV510-Zombie Dye BioLegend Cat# 423102 Fixation Buffer BioLegend Cat# 420801 Intracellular Staining Perm Wash Buffer BioLegend Cat# 421002 Cisplatin Fluidigm Cat# 201064 Maxpar Fix and |